anti ccl2 blocking ab  (Bio X Cell)

Journal: JCI insight

Article Title: A positive cytokine/chemokine feedback loop establishes plasmacytoid DC-driven autoimmune pancreatitis in IgG4-related disease.

doi: 10.1172/jci.insight.167910

Figure Lengend Snippet: Figure 5. Expression of CCR9 on pDCs in MRL/MpJ mice treated with repeated injections of poly(I:C). MRL/MpJ mice were treated with or without poly(I:C), as indicated in Figure 3. After sacrifice at 3 hours following the final set of injections, pancreases were removed and analyzed as indicated. (A) Flow cytometric analysis of cell membrane expression of CCR2, CCR7, and CCR9 on pDCs in pancreatic mononuclear cells extracted from MRL/MpJ mice treated with 16 doses of poly(I:C). Analysis gate was set on PDCA-1+B220lo pDCs. Black curve: control Ab. Red curve: CCR2, CCR7, and CCR9 Abs. Results shown are representative data from 3 mice. (B) Concentrations of CCL2, CCL21, and CCL25 in protein extracts of pancreatic tissues obtained from untreated mice (No Tx, n = 10) or mice treated with 16 injections of poly(I:C) (n = 10), as determined by ELISA. Each dot represents the value derived from 1 mouse. Statis- tical analyses were performed using an unpaired, 2-tailed Student’s t test. Results shown are combined data of 2 independent experiments (B). Results are expressed as mean ± SEM. **P < 0.01.

Article Snippet: Briefly, in some studies, MRL/MpJ mice were administered anti-IFNAR Ab (100 μg, BD Biosciences), anti–IL-33 1 7 R E S E A R C H A R T I C L E JCI Insight 2024;9(20):e167910 https://doi.org/10.1172/jci.insight.167910 receptor ST2 Ab (anti-ST2 Ab, 100 μg, R&D Systems), or anti–bone marrow stromal cell Ag 2 Ab (120G8 Ab, 100 μg, Dendritics) prior to administration of each poly(I:C) injection, whereas in other studies mice were administered anti-CXCR3 Ab (200 μg, Bio X Cell), anti-CCL2 Ab (100 μg, Bio X Cell), or anti-CCL25 Ab (100 μg, R&D Systems) prior to each poly(I:C) injection.

Techniques: Expressing, Membrane, Control, Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: JCI insight

Article Title: A positive cytokine/chemokine feedback loop establishes plasmacytoid DC-driven autoimmune pancreatitis in IgG4-related disease.

doi: 10.1172/jci.insight.167910

Figure Lengend Snippet: Figure 6. Pancreatic accumulation of pDCs does not require the molecular interaction between CCR2 and CCL2. MRL/MpJ mice were administered poly(I:C) twice a week for a total of 16 times. Each poly(I:C) injection was preceded by intraperitoneal injection of anti-CCL2 Ab (200 μg, n = 5) or control Ab (200 μg, n = 5). After sacrifice at 3 hours following the final set of injections, pancreases were removed and analyzed as indicated. (A and B) Representative hematoxylin and eosin staining of the pancreatic tissues and pathological scores of induced autoimmune pancreatitis in the 2 groups. Original magnification, ×400. (C) Concentrations of IFN-α, IL-33, TNF-α, and IL-6 in protein extracts of pancreatic tissues obtained from mice in the 2 groups. (D) Flow cytometric analyses showing the percentages of pDCs, CD3+ T cells, CD11b+ myeloid cells, and CD11c+ DCs among pancreatic mononuclear cells. Left panels: Representative flow cytometric analyses. Right panels: Bar graphs of cumulative data from individual mice. pDCs were defined as PDCA-1+B220lo cells. Each dot represents the value derived from 1 mouse. Statistical analyses were performed using an unpaired, 2-tailed Student’s t test. Results are expressed as mean ± SEM. **P < 0.01.

Article Snippet: Briefly, in some studies, MRL/MpJ mice were administered anti-IFNAR Ab (100 μg, BD Biosciences), anti–IL-33 1 7 R E S E A R C H A R T I C L E JCI Insight 2024;9(20):e167910 https://doi.org/10.1172/jci.insight.167910 receptor ST2 Ab (anti-ST2 Ab, 100 μg, R&D Systems), or anti–bone marrow stromal cell Ag 2 Ab (120G8 Ab, 100 μg, Dendritics) prior to administration of each poly(I:C) injection, whereas in other studies mice were administered anti-CXCR3 Ab (200 μg, Bio X Cell), anti-CCL2 Ab (100 μg, Bio X Cell), or anti-CCL25 Ab (100 μg, R&D Systems) prior to each poly(I:C) injection.

Techniques: Injection, Control, Staining, Derivative Assay